ABSTRACT
Abstract Pitted keratolysis is a skin disorder that affects the stratum corneum of the plantar surface and is caused by Gram-positive bacteria. A 30-year-old male presented with small punched-out lesions on the plantar surface. A superficial shaving was carried out for scanning electron microscopy. Hypokeratosis was noted on the plantar skin and in the acrosyringium, where the normal elimination of corneocytes was not seen. At higher magnification (x 3,500) bacteria were easily found on the surface and the described transversal bacterial septation was observed.
Subject(s)
Humans , Male , Adult , Skin Diseases, Bacterial/pathology , Corynebacterium Infections/pathology , Foot Dermatoses/microbiology , Foot Dermatoses/pathology , Microscopy, Electron, Scanning , Epidermis/pathology , Keratosis/microbiology , Keratosis/pathologyABSTRACT
Objective: The efficacy of topical natamycin 5% was studied using a reproducible model of keratomycosisproduced by Candida albicans in the rabbits. Method: Candida albicans was isolated from infected human eye and 4 x 105 cells of the Candida albicans was injected into the corneal stroma of the eyes of 15 rabbits. All eyes developed a corneal ulcer without pretreatment with immunosuppressive agents. Forty-eight hours after inoculation, the animals were divided into two groups: test group I, 10 eyes receiving natamycin drops in a 5% suspension; control group II, five eyes receiving 0.9% normal saline solution. The rabbits¡¯ corneas were removed for Candida albicans recovery and placed in 1 ml of sterile 0.9% normal saline solution, minced within two hours with scalpel and thoroughly homogenized with a piston and mortar. Serial dilutions of this corneal solution from 10-1 ¨C 10-4 were made in 0.9% sterile saline solution and 100 ¦Ìlaliquots were plated onto tryptic soy agar. All cultures of cornea from the treated eyes were negative after seven days of inoculation while five cultures were still positive in the control eyes at the end of the experiment. Result: It was found that 5% natamycin was effective in treating experimental Candida albicans induced keratomycosis in rabbits. Conclusion: It is concluded that natamycin has a significant effect (p < 0.01) against Candida albicansin treating experimental keratomycosis.
Objetivo: La eficacia de la natamicina tópica al 5% fue estudiada usando un modelo reproducible de queratomicosis producida por Candida albicans en conejos. Método: Candida albicans fue aislada de una infección ocular humana y 4 x 105 células de Candida albicans fueron inyectadas en el estroma córneo de los ojos de 15 conejos. Todos los ojos desarrollaron una úlcera córnea sin pre-tratamiento con agentes inmunosupresores. Cuarenta y ocho horas después de la inoculación, los animales fueron divididos en dos grupos: un grupo experimental I, en el que diez (10) ojos recibieron gotas de natamicina en suspensión al 5%; y un grupo control II, en el que cinco(5) ojos recibieron solución salina normal al 0.9%. Las córneas de los conejos fueron extraídas para recuperar Candida albicans y colocadas en 1 ml de solución salina normal estéril, para ser luego desmenuzadas a las dos horas con un escalpelo, y homogeneizadas completamente con un mortero. Sehicieron diluciones seriadas de esta solución córnea de 10-10 -10-4 en solución salina al 0.9% y 100 mlde alícuotas fueron colocadas en placas con agar de soya tríptico. Todos los cultivos de corneas de los ojos tratados, resultaron negativos luego de siete días de inoculación, mientras que 5 cultivos eran todavía positivos al final del experimento en los ojos del control.Resultado: Se halló que la natamicina al 5% era efectiva en el tratamiento de la queratomicosis inducida experimentalmente mediante Candida albicans en conejos. Conclusión: Se concluyó que la natamicina surte efecto (p < 0.01) sobre Candida albicans en eltratamiento experimental de la queratomicosis.
Subject(s)
Animals , Rabbits , Candida albicans , Keratosis/drug therapy , Keratosis/microbiology , Natamycin/therapeutic use , Models, Animal , Keratosis/complicationsABSTRACT
Se presentan los datos clínicos, epidemiológicos, histopatológicos y microbiológicos de 100 varones jóvenes con queratólisis punteada de localización plantar. La dermatosis fue bilateral en 97 porciento, todos presentaron depresiones puntiformes y diferentes cambios de color. Por histopatología en 90 por ciento se demostraron filamentos y elementos bacilares y cocoides en capa córnea, más evidentes con PAS y Gomori-Grocott. En 46 porciento se aisló Micrococcus sedentarius. Se presenta este microorganismo como probable agente etiológico asociado a una flora bacteriana abundante y como factores predisponentes humedad, fricción y maceración.
Subject(s)
Humans , Male , Adolescent , Keratosis/microbiology , Micrococcus/isolation & purification , Causality , Cross-Sectional Studies , Epidemiology, Descriptive , Keratosis/epidemiology , Keratosis/pathology , Mexico , Prospective StudiesABSTRACT
Three morphological varieties of hyperkeratotic and verrucous skin lesions on the anterior aspect of ankle joints in patients with leprosy are described: (i) verrucous lesions with thread-like horny projections similar to filiform warts; (ii) irregular compact hyperkeratotic lesions with deep fissures in between; and (iii) hyperkeratotic lesions with linear fissures corresponding to the transverse creases on the anterior aspect of the ankle. Chemical cautery was useful for the treatment of the first two varieties, and a potent topical corticosteroid with salicylic acid was useful for the third.